昆虫取食诱导的蒙古沙冬青细胞跨膜离子流对代谢组的影响

项目名称： 昆虫取食诱导的蒙古沙冬青细胞跨膜离子流对代谢组的影响

项目编号： No.31270655

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 农业科学

项目作者： 沈应柏

作者单位： 北京林业大学

项目金额： 85万元

中文摘要： 植物识别外界刺激早期信号事件的关键研究对象之一是跨膜离子流。然而，跨膜离子流如何调控下游变化，从而使植物对外界刺激做出特定响应的机理目前还不清楚。由于植物识别昆虫取食机理研究的滞后，影响了国家三级濒危植物蒙古沙冬青的保护和开发利用。本项目以沙冬青和其主要害虫灰斑古毒蛾为实验材料，利用非损伤微测技术、激光共聚焦显微技术和基于核磁共振（NMR）的代谢组学技术，创新性地将植物识别外界刺激的早期信号事件跨膜离子流变化与植物的代谢组变化结合起来，分析植物识别昆虫口腔分泌物和茉莉酸甲酯过程中，胞内Ca2+不同来源和胞质pH变化对代谢组的影响，建立此过程中不同Ca2+来源和胞质pH变化调控代谢途径的代谢网络图。揭示细胞跨膜Ca2+和H+离子流在植物细胞识别昆虫取食和调控代谢变化中的作用。阐明植物对昆虫取食的识别和代谢响应机理。为开发沙冬青虫害的新型防治方法提供理论依据。

中文关键词： 昆虫取食；离子流；信号转导；茉莉酸；代谢组

英文摘要： Ion fluxes across membrane are crucial signals in the response of plants to external stimuli. However, the method that the ion fluxes across membrane use to regulate downstream events remains unknown. Because of the lag in this regulation mechanism, the population of Ammopiptanthus mongolicus keeps decreasing for the negative effect of insects. In order to find out the function of Ca2+ and H+ in signal transduction and metabolism regulation in plants in the perception of herbivore so as to shed some light on the mechanism of perception in plant to insects and explore new ways to protect Ammopiptanthus mogolicus against insects, ion fluxes across membrane and the induced metabolic change would be combined innovatively. Different Ca2+ and H+ sources on the metabolism of A. mogolicus cells which were excited by insect oral secretion or methyl jasmonate will be examind with Non-invasive Micro-test technology, Laser Scanning Confocal Microscope and Nuclear Magnetic Resonance based metabolomics technology and a metabolic network which were influenced by different Ca2+ and H+ sources in this process would also be establish.

英文关键词： herbivory；ion flux；signal transduction；jasmonate；metabolome