Foxl2在三疣梭子蟹卵巢发育中的作用及其机制研究

项目名称： Foxl2在三疣梭子蟹卵巢发育中的作用及其机制研究

项目编号： No.41306178

项目类型： 青年科学基金项目

立项/批准年度： 2014

项目学科： 天文学、地球科学

项目作者： 孟宪亮

作者单位： 中国水产科学研究院黄海水产研究所

项目金额： 25万元

中文摘要： 叉头框蛋白L2（Forkhead box L2，Foxl2）是在卵巢发育中发挥重要调控作用的一种转录因子。本项目在已获得的三疣梭子蟹Foxl2及其下游靶基因序列片段的基础上，克隆这些基因的全长序列；从mRNA和蛋白水平分析Foxl2在三疣梭子蟹卵巢发育过程中的表达特征；通过检测RNA干扰Foxl2表达对卵巢发育的影响，分析Foxl2在卵巢发育中的作用；明确Foxl2与其靶基因的相互作用，分析Foxl2与其靶基因在三疣梭子蟹卵巢发育过程中表达模式的相关性，并研究干扰Foxl2对其靶基因表达的影响，从而阐明Foxl2在卵巢发育中发挥作用的机制。本项目针对转录因子开展研究，尝试寻找三疣梭子蟹卵巢发育分子调控机制研究的新切入点。预期的研究结果对于深入理解三疣梭子蟹等甲壳动物卵巢发育的分子调控机制具有重要意义，为解决池塘养殖三疣梭子蟹卵巢发育不良的问题奠定理论基础。

中文关键词： 三疣梭子蟹；卵巢发育；转录因子；叉头框蛋白 L2；

英文摘要： Forkhead box L2（Foxl2）is a transcription factor, which plays a critical role in regulating the ovarian development of animals. In our previous study, we have obtained the partial cDNA sequences of Foxl2 and its target genes of the swimming crab, Portunus trituberculatus. The current project will investigate the role and mechanism of Foxl2 in the ovarian development of female P. trituberculatus. Firstly, we will clone and sequence Foxl2 and its target genes, and determine the expression pattern of Foxl2 at mRNA and protein levels during the ovarian development. Secondly, to determine the role of Foxl2 in P.trituberculatus ovarian development, we will examine the effects of knocking down Foxl2 expression on the ovarian development. And then, we will verify the interaction between Foxl2 and its target genes, and clarify the correlation between the expression of Foxl2 and its target genes during the ovarian development. Finally, we will elucidate the regulatory mechanism of Foxl2 in P.trituberculatus ovarian development, by analyzing how Foxl2 knockdown affects the expression of its target genes. This research focuses on an important transcription factor to find a new way of investigating the molecular mechanisms of P.trituberculatus ovarian development. The expected outcomes of this research would not only improve

英文关键词： Portunus trituberculatus；Ovarian development；Transcription factor；Forkhead box protein L2；