基于分子信标自由末端多位点现场标记信号分子的新型DNA传感器

项目名称： 基于分子信标自由末端多位点现场标记信号分子的新型DNA传感器

项目编号： No.21275127

项目类型： 面上项目

立项/批准年度： 2013

项目学科： 数理科学和化学

项目作者： 汪庆祥

作者单位： 闽南师范大学

项目金额： 80万元

中文摘要： 基于分子信标的DNA杂交传感技术与传统直线型DNA探针传感器相比，具有特异性强、选择性高、操作简单等优点。但目前该技术还存在信号分子标记-分离过程复杂、探针信号种类单一、信号强度有限等缺点，限制了该类传感器的深入研究和广泛应用。 为解决上述不足，本课题拟将"多位点平台搭建"和"信号分子现场标记"技术相结合，创建一系列分子信标传感器制备的新方法。主要工作设想包括：(1) 在分子信标自由末端搭建一个多位点平台，用于多个电活性信号分子的同时标记，并通过信号叠加效应提高该类传感器的信号强度；(2)在固定后的分子信标上进行信号分子的现场标记，通过固液相差异简单实现电活性分子信标"标记-分离-纯化" 的同步完成，克服均液相合成中分离、纯化困难的弊端；(3)将不同类型的多位点平台和信号分子进行搭配和组装，丰富该类传感器的信号类型，并根据不同标记分子的信号差异努力实现传感器对多目标序列的同时检测。

中文关键词： 分子信标；多位点平台；现场组装；电化学传感器；DNA杂交

英文摘要： Compared with the traditional linear probe-based DNA biosensor, the molecular beacon (MB)-based hybridization biosensing technology shows the advantages of stronger specificity, higher selectivity, more simple operability and more comprehensive application. However, there are still some disadvantages for this technology, for instance, the labeling and separating processes for the electroactive MB probe are often complicated, the type of the signal molecule labeled on MB probe is few and signal intensity in the classical MB-based biosensor is limited. To solve these problems, a new strategy that based on the multi-site and in-situ labeling of the electroactive signal molecules on the free terminal of MB probe is established in this project. The main ideas include: (1) Constructing a multi-site platform on the free terminal of the MB probe for several signal molecules to be labeled simultaneously, and therefore the detection signal can be greatly amplified and the analytical sensitivity will be increased. (2) Applying in-situ labeling of the electroactive molecule on the immobilized MB probe to realize the accomplishment of the "labeling-separation-purification" by one step. Thus, the drawbacks of bothering purification steps, time and reagent-consuming that accompanied by the common homogeneous synthesis can be

英文关键词： Molecular beacon；Multi-site platform；In-situ assembly；Electrochemical sensor；DNA hybridization