基于金属纳米簇电致化学发光特性的激酶活性检测研究

项目名称： 基于金属纳米簇电致化学发光特性的激酶活性检测研究

项目编号： No.21475056

项目类型： 面上项目

立项/批准年度： 2015

项目学科： 数理科学和化学

项目作者： 梁汝萍

作者单位： 南昌大学

项目金额： 90万元

中文摘要： 本项目拟针对与重大疾病相关的蛋白激酶，设计和筛选蛋白激酶特异性识别的底物多肽，并以多肽为模板合成具有优良电致化学发光（ECL）特性的金属纳米簇，探讨金属纳米簇的电化学发光机制与构效关系。在研究纳米材料与生物分子相互作用机制的基础上，实现生物分子的定向偶联和有序组装，制备纳米生物复合探针和生物传感界面，研究靶标蛋白激酶与底物多肽的相互作用及其对金属纳米簇光电性质的影响，将该特异性生物识别过程有效转换为金属纳米簇的光电信号变化，获取金属纳米簇与蛋白激酶活性之间的识别传感规律。利用活性氧对金属纳米簇ECL的猝灭、金属纳米簇生物复合探针的ECL增强、纳米材料对金属纳米簇阴极ECL猝灭和对鲁米诺阳极ECL增强等效应，构建基于金属纳米簇ECL信号关、信号开和双信号比率放大等策略的高灵敏性和选择性蛋白激酶活性检测及抑制剂筛选传感平台。

中文关键词： 金属纳米簇；电致化学发光；生物传感器；蛋白激酶活性；抑制剂

英文摘要： In this project, for the analysis of protein kinases associated with critical diseases, the substrate peptides which are targeted by the specific protein kinases are screened and designed, and used as templates and stablizers to synthesize electrochemiluminescent metal nanoclusters with high luminous efficiency and good stability. The electrogenerated chemiluminescence behavior and mechanism of metal nanoclusters will be studied in detail. The important physical and chemical properties of energy transfer and material transmission are effectively converted into photoelectric signals through the photoelectric effects of nanoclusters in the biomolecular recognition process. Based on this, a series of electrochemiluminescent sensing strategies (signal on, signal off, and dual-signal ratiometric approaches) for highly sensitive assay of protein kinases activity and inhibitions can be developed by taking advantages of cationic mediated, biotin-streptavidine mediated, antibody-antigen mediated and S-Au mediated electrogenerated chemiluminescence signal transition and signal amplification (or signal decrease) of metal nanoclusters. The sensing platform will be further used to detect protein kinases activity in biological samples and screen potential inhibitors, which is of great significance for the development of targeted therapy, clinical diagnosis, and studies of cellular signal transduction pathways.

英文关键词： Metal nanocluster;Electrogenerated chemiluminescence;Biosensor;Protein kinase activity;Inhibitor