钛表面多级微孔结构及其生物学性能

项目名称： 钛表面多级微孔结构及其生物学性能

项目编号： No.50871093

项目类型： 面上项目

立项/批准年度： 2009

项目学科： 生物科学

项目作者： 冯波

作者单位： 西南交通大学

项目金额： 33万元

中文摘要： 本项目基于钛表面多级微结构的构建与表征，评价其生物学性能。材料制备方面，涉及不同管径、不同管长及不同晶体结构的钛表面纳米管；既有微米孔又有纳米管的氧化钛层；数百微米的多孔钛孔壁同时存在纳米管等多种微纳结构层、改性层表面生物活性功能涂层等。较为系统地表征了钛表面氧化钛纳米管层和微纳米多级微孔层的几何尺寸、晶体结构、力学性能等。通过改进的仿生矿化法制备了高结合强度的功能性复合涂层，对钙磷成核结晶、活性涂层与底材结合强度关系进行了分析。初步认识了钛表面纳米管层和微纳米多级微孔层的结构参数对蛋白质和细胞行为的影响机制。通过体内试验及其组织学、分子生物学评价研究了钛表面微纳结构对骨组织生长的影响，探讨其在骨修复方面的应用潜力。采用层层自组装技术对钛表面和氧化钛纳米管层表面进行生物化改性，研究了改性层的结构、理化性能和生物学性能。对多孔钛进行纳米化改性后，采用体内试验研究了骨组织的生长行为和界面结合能力。此外，对于氧化钛纳米管的抗菌性、耐磨减摩性也进行了初步评价。本研究结果在理论方面加深认识了表面微纳米结构对生物医学材料性能的影响机制，可为其临床应用提供一定基础。

中文关键词： 医用钛；表面改性；多级微孔结构；生物学性能；复合涂层

英文摘要： The multi-scaled micro/nano-structures on titanium surfaces were fabricated and their biological properties were evaluated. Modified materials included nanotubes on titanium surfaces with different lengths, diameters or crystal structures; microporous layers with nanotubes; macroporous titanium with nanotubes on the porous walls; biofunctional coatings on the modified titanium. The geometry, crystal phase and mechanical properties were characterized. The influence of the micro/nano-structrues on the biological properties of titanium was studied by in vitro and in vivo experiments based on histology and molecular biology evaluations. The functional coatings with high bond-strength to the micro/nano-structural titanium were prepared by a revised biomineral method. The bioactivity of the nano- and micro/nano-structural titanium enhanced as increase of nanotube diameters within the certain size range. And peptite and collagen grafted or self-assembled were beneficial to increase bioactivity of the micro/nano-structural titanium. The bioactivity of them well related to crystal structure of the micro/nano-structural layers on titanium. In the early implantation, the nano-structural titanium had better bone-growth ability than the micro/nano-structural titanium. As time extended, both of them showed similar bone-gowth ability and was better than flat titanium. Titania nanotubes on titanium surfaces possessed antibacterial properites and good wear-resistence. Our results contribute to understand the interaction mechanism of biological tissue and micro/nano structure of tianium, and can provide the theoretical basis to clinics.

英文关键词： Titanium in medicine; surface modification; Multi-scale miroporous structure; biological properties; composite coating